A METALLOTHIONEIN-LIKE GENE FROM MAIZE (ZEA-MAYS) - CLONING AND CHARACTERIZATION

被引:73
|
作者
DEFRAMOND, AJ
机构
[1] Department of Plant Molecular Biology, CIBA-Geigy Agricultural Biotechnology Research Unit, Research Triangle Park, NC 27709-2257
关键词
PLANT METALLOTHIONEIN; METALLOTHIONEIN GENE; 5' FLANKING SEQUENCE; DIFFERENTIAL EXPRESSION; ZEA-MAYS;
D O I
10.1016/0014-5793(91)81236-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A differentially expressed maize gene has been cloned and sequenced. Transcriptional and translational start sites have been mapped and 2.5 kb of 5' flanking DNA were sequenced. The 8 kDa protein encoded by this gene shows striking similarity to the metallothionein-like proteins recently described in Pisum sativum [1] and Mimulus guttatus [2]. The maize MT-L gene message is very abundant in roots without exposure to high levels of metals, present at lower concentration in leaves and pith, and at very low concentration in seed.
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页码:103 / 106
页数:4
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