Plant Assays for Quantifying Ralstonia solanacearum Virulence

被引:24
|
作者
Khokhani, Devanshi [1 ]
Tuan Minh Tran [2 ]
Lowe-Power, Tiffany M. [3 ]
Allen, Caitilyn [4 ]
机构
[1] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA
[2] Nanyang Technol Univ, Sch Biol Sci, Singapore, Singapore
[3] Univ Calif Berkeley, Plant & Microbial Biol, Berkeley, CA 94720 USA
[4] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA
来源
BIO-PROTOCOL | 2018年 / 8卷 / 18期
关键词
Virulence assay; Bacterial wilt; Vascular plant pathogen; Plant colonization; Xylem sap; R; solanacearum; Tomato;
D O I
10.21769/BioProtoc.3028
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Virulence assays are powerful tools to study microbial pathogenesis in vivo. Good assays track disease development and, coupled with targeted mutagenesis, can identify pathogen virulence factors. Disease development in plants is extremely sensitive to environmental factors such as temperature, atmospheric humidity, and soil water level, so it can be challenging to standardize conditions to achieve consistent results. Here, we present optimized and validated experimental conditions and analysis methods for nine assays that measure specific aspects of virulence in the phytopathogenic bacterium Ralstonia solanacearum, using tomato as the model host plant.
引用
收藏
页数:19
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