DETERMINATION OF GLYCOSYLATION SITES IN O-LINKED GLYCOPEPTIDES - A SENSITIVE MASS-SPECTROMETRIC PROTOCOL

被引：25
|
作者：
RADEMAKER, GJ
HAVERKAMP, J
THOMASOATES, J
机构：
[1] Bijvoet Center for Biomolecular Research, Department of Mass Spectrometry, Utrecht University, Utrecht, 3508 TB
来源：
ORGANIC MASS SPECTROMETRY | 1993年 / 28卷 / 12期
关键词：
D O I：
10.1002/oms.1210281228
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A sensitive mass spectrometric method for the specific identification of O-glycosylation sites in glycopeptides is presented. Reductive beta-elimination liberates a peptide containing a specifically modified amino acid at the former site of carbohydrate attachment. Mass spectrometry is used to determine the sequence of the modified peptide, in which the position of the modified amino acid marks the site of O-glycosylation. The method also provides information on the carbohydrate chain and is applicable at the low nanomolar level.
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页码：1536 / 1541
页数：6
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