DETERMINATION OF GLYCOSYLATION SITES IN O-LINKED GLYCOPEPTIDES - A SENSITIVE MASS-SPECTROMETRIC PROTOCOL

被引：25
|
作者：
RADEMAKER, GJ
HAVERKAMP, J
THOMASOATES, J
机构：
[1] Bijvoet Center for Biomolecular Research, Department of Mass Spectrometry, Utrecht University, Utrecht, 3508 TB
来源：
ORGANIC MASS SPECTROMETRY | 1993年 / 28卷 / 12期
关键词：
D O I：
10.1002/oms.1210281228
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A sensitive mass spectrometric method for the specific identification of O-glycosylation sites in glycopeptides is presented. Reductive beta-elimination liberates a peptide containing a specifically modified amino acid at the former site of carbohydrate attachment. Mass spectrometry is used to determine the sequence of the modified peptide, in which the position of the modified amino acid marks the site of O-glycosylation. The method also provides information on the carbohydrate chain and is applicable at the low nanomolar level.
引用
收藏
页码：1536 / 1541
页数：6
相关论文
共 50 条
  • [1] DETERMINATION OF N-LINKED AND O-LINKED CARBOHYDRATES AND ATTACHMENT SITES WITH HIGH-SENSITIVITY - ADVANCED MASS-SPECTROMETRIC METHODS
    MEDZIHRADSZKY, KF
    SETTINERI, CA
    YU, Z
    SCHINDLER, PA
    BURLINGAME, AL
    GLYCOCONJUGATE JOURNAL, 1993, 10 (04) : 276 - 276
  • [2] Mass spectrometric analysis, automated identification and complete annotation of O-linked glycopeptides
    Darula, Zsuzsa
    Chalkley, Robert J.
    Baker, Peter
    Burlingame, Alma L.
    Medzihradszky, Katalin F.
    EUROPEAN JOURNAL OF MASS SPECTROMETRY, 2010, 16 (03) : 421 - 428
  • [3] STRUCTURE DETERMINATION OF O-LINKED GLYCOPEPTIDES BY TANDEM MASS-SPECTROMETRY
    MEDZIHRADSZKY, KF
    GILLECECASTRO, BL
    SETTINERI, CA
    TOWNSEND, RR
    MASIARZ, FR
    BURLINGAME, AL
    BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1990, 19 (12): : 777 - 781
  • [4] Decoding of O-Linked Glycosylation by Mass Spectrometry
    Mulagapati, SriHariRaju t
    Koppolu, Veerendra
    Raju, T. Shantha
    BIOCHEMISTRY, 2017, 56 (09) : 1218 - 1226
  • [5] CNS active O-linked glycopeptides
    Jones, Evan M.
    Polt, Robin
    FRONTIERS IN CHEMISTRY, 2015, 3
  • [6] Localization and structural analysis of the O-linked glycosylation sites of ENBREL® (etanercept) by Tandem Mass Spectrometry
    Balland, A
    Boyce, J
    Jones, P
    Koffley, C
    Goetze, A
    Wang, W
    JOURNAL OF BIOTECHNOLOGY, 2005, 118 : S19 - S19
  • [7] O-linked glycosylation in focus
    Van den Steen, PE
    Rudd, PM
    Wormald, MR
    Dwek, RA
    Opdenakker, G
    TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY, 2000, 12 (63) : 35 - 49
  • [8] Determination of O-Glycosylation Heterogeneity Using a Mass-Spectrometric Method Retaining Sugar Modifications
    Manri, Naomi
    Takegawa, Yasuhiro
    Fujitani, Naoki
    Kaneko, Akihito
    Hirabayashi, Atsumu
    Nishimura, Shin-Ichiro
    Sakamoto, Takeshi
    ANALYTICAL SCIENCES, 2012, 28 (07) : 723 - 727
  • [9] Principal component analysis of O-linked glycosylation sites in protein sequence
    Yang, Xue-Mei
    Chen, Yen-Wei
    Ito, Masahiro
    Nishikawa, Ikuko
    2007 THIRD INTERNATIONAL CONFERENCE ON INTELLIGENT INFORMATION HIDING AND MULTIMEDIA SIGNAL PROCESSING, VOL 1, PROCEEDINGS, 2007, : 121 - +
  • [10] AMINO-ACID DISTRIBUTIONS AROUND O-LINKED GLYCOSYLATION SITES
    WILSON, IBH
    GAVEL, Y
    VONHEIJNE, G
    BIOCHEMICAL JOURNAL, 1991, 275 : 529 - 534
12345