DEVELOPMENT, ESTIMATION AND VALIDATION OF GLIMEPIRIDE IN PHARMACEUTICAL FORMULATION BY HPLC METHOD

The study is focused on developing a simple, rapid, validated High Performance Liquid Chromatographic ( HPLC) method for Glimepiride Tablets from their dosage forms. The detection was carried out at 230 nm using Shimadzu UV - Visible detector HPLC system. The accuracy and precision were determined and validated statistically. The linearity was observed in the range of 15-120 mu g/mL with a correlation coefficient of 0.999. The limit of detection and the limit of quantification were found to be 4 ng and 10 ng respectively. A Lichrosorb (R) (RP-18 column with a mobile phase consisting of acetonitrile - water - glacial acetic acid (550:450:0.6 v/v)) was used. The flow rate was 1 mL/min. The HPLC method is selective, precise and accurate and can be used for routine analysis of preparations in pharmaceutical industry quality control laboratories.