OPTIMIZATION OF FERMENTATION CULTURE CONDITIONS FOR ALKALINE PROTEASE PRODUCTION BY Scopulariopsis spp.

被引：0
|
作者：
Niyonzima, Francois N. [1 ]
More, Sunil S. [1 ]
机构：
[1] Jain Univ, Ctr Post Grad Studies, Dept Biochem, 18-3 9th Main 3rd Block, Bangalore 560011, Karnataka, India
来源：
APPLIED BIOLOGICAL RESEARCH | 2013年 / 15卷 / 01期
关键词：
Alkaline protease; casein; optimization; Scopulariopsis; submerged fermentation;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The present study was aimed to assess alkaline protease production by a locally isolated soil fungus Scopulariopsis spp. The factors influencing enzyme production were optimized under submerged conditions. Maximum enzyme production occurred on 5th day of incubation on fermentation medium with initial pH 9.0, incubation temperature 379 degrees C, inoculum concentration 3%, casein 2% as substrate and 1.5% tryptone as nitrogen source. Under these optimized conditions, about 3-fold increase in protease activity ( from 5.85 to 17.77 U ml(-1)) was noticed. High enzyme activity at alkaline pH suggested that the fungus can be exploited for use in leather processing and detergent industries.
引用
收藏
页码：66 / 69
页数：4
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