DIRECT SOMATIC EMBRYOGENESIS AND PLANT-REGENERATION FROM PROTOPLAST-DERIVED CELLS OF WHEAT (TRITICUM-AESTIVUM L)

Protoplasts were isolated from embryogenic calli derived from immature embryos of wheat (Triticum aestivum L. cv. Jinan 177). The protoplasts were cultured in NMB medium supplemented with 1mg/1 2,4-D and 500mg/1 casein hydrolysate (CH). The regenerated cells from protoplasts divided to form somatic embryos directly. The somatic embryos grown to 1.5-2 mm in size directly developed into complete plants on solid MB medium without hormones.