Anti Stress Activity (in-vivo) of Forskolin Isolated from Coleus forskohlii

Coleus forskohlii (Labatiae) is a plant widely used as food and medicine worldwide but isolation of active compound (Forsklin) from hydroalcoholic extract and antistress activity of Forskolin is not reported. Dried and powder rhizome of Coleus forskohlii (Labatiae) was used to isolate the chemical constituent using phase column chromatography and TLC purification. Elevated plus maze (EPM) model and forced swimming test (FST) were performed to assess antistress activity on albino mice. (Doses of the isolated chemical constituents: 25, 50 and 100 mg/kg orally) The structure of the isolated compound was determined by FTIR, 1H NMR and Mass spectroscopy. The results of elevated plus maze (EPM) model and forced swimming test (FST) revealed that isolated compound having antistress activity. This is the first report of antistress activity of Forskolin and isolation by column chromatography separation, and TLC purification. The result also revealed that Forskolin is a novel compound for the treatment of neurobiological disorder (stress). A scientific investigation of traditional herbal remedies (Forskolin) for stress may provide valuable leads for the development of alternative drugs and therapeutic strategies.