Anti-MV Diterpenes from Coleus forskohlii

Various extracts of the aerial parts of Coleus forskohlii (Labiatae) were prepared and evaluated at their non cytotoxic concentration against HIV-1 (NL4-3). Chloroform, ethyl acetate and n-butanol extracts showed 45.6, 66.5 and 37.7%, inhibition of HIV, respectively in CEM-GFP cells infected with HIV-I(NL4-3) at 5 mu g/mL. Four diterpenes, 1-deoxyforskolin, 1,9-dideoxyforskolin, forskolin and isoforskolin were isolated from the chloroform extract and tested against the virus. Six semi-synthetic derivatives of forskolin have been prepared to study SAR. 1-Deoxyforskolin and forskolin were found to be active against HIV(NL4-3). This is first report of anti HIV activity of this plant and its isolated constituents.