METHODS USED FOR ANALYSES OF ENVIRONMENTALLY DAMAGED NUCLEIC-ACIDS

被引：21
|
作者：
FRENKEL, K
KLEIN, CB
机构：
[1] NYU MED CTR,DEPT PATHOL,NEW YORK,NY 10016
[2] NYU MED CTR,KAPLAN COMPREHENS CANC CTR,NEW YORK,NY 10016
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 618卷 / 1-2期
关键词：
D O I：
10.1016/0378-4347(93)80039-7
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In this review, we present various techniques, currently applied in many laboratories, which are useful in the detection of ''environmentally''-induced damage to DNA. These techniques include: (a) chromatographic methods, which allow determination of chemical changes within DNA, be they formation of adducts with or oxidation of bases in DNA; (b) electrophoretic methods, which facilitate finding the site(s) in DNA where that chemical modification occurred; and (c) immunological assays, which help to detect DNA damage using externally produced antibodies that recognize the specific chemical changes in DNA or its fragments, as well as by detection of autoantibodies that develop in response to environmental exposures of animals and humans.
引用
收藏
页码：289 / 314
页数：26
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