THE HEMIMETHYLATED REPLICATION ORIGIN OF ESCHERICHIA-COLI CAN BE INITIATED INVITRO

被引：19

作者：

BOYE, E

机构：

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 14期

关键词：

D O I：

10.1128/JB.173.14.4537-4539.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Unmethylated, fully methylated, and hemimethylated oriC-containing plasmids were assayed as substrates for DNA replication in vitro by using a system reconstituted with pure proteins. In contrast to the in vivo situation, all three substrates were initiated efficiently; the fully methylated plasmid was about twice as active as the other two.

引用

页码：4537 / 4539

页数：3

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