LIQUID-CHROMATOGRAPHY DETERMINATION OF SIMAZINE AND ANTIMYCIN-A IN MUST

A method for extraction and determination of trace amounts of simazine and four antimycins A in must is described. A 5.0 mL sample was extracted in two portions of 3.0 mL of diethyl ether at pH 5.0. After removal of the solvent, the extracted sample was dissolved in 1.0 mL of 20 mM acetic acid/sodium acetate aqueous solution (pH 5)-methanol (20:80) and analyzed by liquid chromatography with UV detection at 230 nm. Recoveries of simazine and antimycin A at the 0.45-3.0 mg L(-1) level were between 79 and 106% with standard deviation between 2.3 and 4.5% (n = 8). Detection limits for analytes in must were 20, 90, 115, 50, and 45 mu g L(-1) for simazine, antimycin A(1), antimycin A(2), antimycin A(3), and antimycin A(4), respectively.