DETERMINATION OF AMPICILLIN IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING ULTRAVIOLET DETECTION

被引：14
|
作者：
AKHTAR, MJ [1 ]
KHAN, S [1 ]
KHAN, MAS [1 ]
机构：
[1] WELLCOME PAKISTAN LTD,RES & DEV LABS,D-43,SITE,KARACHI,PAKISTAN
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1993年 / 11卷 / 4-5期
关键词：
AMPICILLIN; REVERSED-PHASE HPLC; ISOCRATIC ELUTION; UV DETECTION; HUMAN PLASMA;
D O I：
10.1016/0731-7085(93)80031-U
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A high-performance liquid chromatographic method with isocratic elution has been developed for the determination of ampicillin in human plasma. The method comprises injection of the plasma after protein precipitation on to a reversed-phase C18 column, using phosphate buffer (pH 3.5)-methanol as the mobile phase. The method involves UV detection at 225 nm. This method provides a simple technique for the rapid analysis of ampicillin within the concentration range 0.2-25 mug ml-1 with a high degree of accuracy and precision.
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页码：375 / 378
页数：4
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