PURIFICATION AND SOME PROPERTIES OF BETA-MANNANASE FROM BACILLUS SP

被引：21
|
作者：
OOI, T
KIKUCHI, D
机构：
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1995年 / 11卷 / 03期
关键词：
BACILLUS SP; MANNAN; BETA-MANNANASE;
D O I：
10.1007/BF00367106
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
beta-Mannanase produced by Bacillus sp. W-2, isolated from decayed commercial konjak cake, was purified from the culture supernatant by (NH4)(2)SO4 precipitation, adsorption to konjak gel, and column chromatography with DEAE-cellulose, Sephadex G-100 and Sephacryl S-200. Its molecular size was estimated by SDS-PAGE as 40 kDa, and by gel filtration as 36 kDa. The enzyme was most active at pH 7 and 70 degrees C and was stable for at least 1 h between pH 5 and 10 and below 60 degrees C. Its activity was completely inhibited by Hg2+. The enzyme hydrolysed galactomannan better than glucomannan and mainly produced mannose and mannobiose.
引用
下载
收藏
页码：310 / 314
页数：5
相关论文
共 50 条
  • [21] CLONING AND EXPRESSION OF BETA-MANNANASE FROM TRICHODERMA-REESEI
    STALBRAND, H
    SALOHEIMO, A
    VEHMAANPERA, J
    HENRISSAT, B
    PENTTILA, M
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1995, 209 : 195 - BIOT
  • [22] PURIFICATION AND SOME PROPERTIES OF ENDO-1,4-BETA-D-MANNANASE FROM PSEUDOMONAS SP PT-5
    YAMAURA, I
    MATSUMOTO, T
    FUNATSU, M
    FUNATSU, Y
    AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1990, 54 (09): : 2425 - 2427
  • [23] PURIFICATION AND SOME PROPERTIES OF A NEW LEVANASE FROM BACILLUS SP NO 71
    MURAKAMI, H
    KURAMOTO, T
    MIZUTANI, K
    NAKANO, H
    KITAHATA, S
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1992, 56 (04) : 608 - 613
  • [24] THE BETA-MANNANASE FROM CALDOCELLUM-SACCHAROLYTICUM IS PART OF A MULTIDOMAIN ENZYME
    GIBBS, MD
    SAUL, DJ
    LUTHI, E
    BERGQUIST, PL
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (12) : 3864 - 3867
  • [25] PURIFICATION AND CHARACTERIZATION OF A BETA-MANNANASE FROM THE DIGESTIVE-TRACT OF THE EDIBLE SNAIL HELIX-LUCORUM L
    FLARI, V
    MATOUB, M
    ROULAND, C
    CARBOHYDRATE RESEARCH, 1995, 275 (01) : 207 - 213
  • [26] Simple Laboratory Exercise for Induction of beta-Mannanase from Aspergillus niger
    Mulimani, V.
    Naganagouda, K.
    JOURNAL OF FOOD SCIENCE EDUCATION, 2010, 9 (03): : 76 - 79
  • [27] SPECIFICITY OF BETA-MANNANASE FROM PENICILLIUM-PURPUROGENUM FOR KONJAC GLUCOMANNAN
    KUSAKABE, I
    PARK, GG
    KUMITA, N
    YASUI, T
    MURAKAMI, K
    AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1988, 52 (02): : 519 - 524
  • [28] STUDIES ON THE MANNANASE OF STREPTOMYCES .4. STRUCTURES OF GLUCOMANNO-OLIGOSACCHARIDES FROM THE HYDROLYTIC PRODUCTS OF KONJAC GLUCOMANNAN PRODUCED BY A BETA-MANNANASE FROM STREPTOMYCES SP
    TAKAHASHI, R
    KUSAKABE, I
    KUSAMA, S
    SAKURAI, Y
    MURAKAMI, K
    MAEKAWA, A
    SUZUKI, T
    AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1984, 48 (12): : 2943 - 2950
  • [29] Purification and characterization of extremely thermostable beta-mannanase, beta-mannosidase, and alpha-galactosidase from the hyperthermophilic eubacterium Thermotoga neapolitana 5068
    Duffaud, GD
    McCutchen, CM
    Leduc, P
    Parker, KN
    Kelly, RM
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (01) : 169 - 177
  • [30] Partial purification and some properties of an alkaline cellulase from an alkalophilic Bacillus sp.
    KhyamiHorani, H
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1996, 12 (05): : 525 - 529
12345