INTRAVENOUSLY ADMINISTERED (1-])-BETA-D-GLUCAN, SSG, OBTAINED FROM SCLEROTINIA-SCLEROTIORUM IFO 9395 AUGMENTS MURINE PERITONEAL MACROPHAGE FUNCTIONS INVIVO

Effect of intravenously (i.v.) or intraperitoneally (i.p.) administered (1 --> 3)-beta-D-glucan, SSG, obtained from Sclerotinia sclerotiorum IFO 9395 on the murine peritoneal macrophage (PM) functions were examined. A single i.v. administration of SSG increased the number of PMs at a dose of 250-mu-g/mouse, and the peak appeared 4d after administration. However, no special change was observed on peritoneal exude cell (PEC) populations. These PMs showed augmented lysosomal enzyme activity and the peaks appeared in 2 phases, on days 2 and 10. In contrast, SSG administered i.p. (250-mu-g/mouse) increased the number of PMs and enhanced the lysosomal enzyme activity of PMs from day 4, and a broad peak appeared until days 8-12. The populations of PECs were also changed by i.p. injection of SSG. Additionally, SSG administered i.v. enhanced phagocytic activity, H2O2 production and interleukin 1 (IL-1) production, and the kinetics of the activation differed depending on the activities. These data suggest that the effects of SSG on macrophage functions are different depending on administration routes, and there are some different mechanisms in the activation of macrophages in vivo hy SSG.