AMPLIFIED COLORIMETRIC ASSAY OF ALKALINE-PHOSPHATASE USING RIBOFLAVIN 4'-PHOSPHATE - A SIMPLE METHOD FOR MEASURING RIBOFLAVIN AND RIBOFLAVIN 5'-PHOSPHATE

被引：9
|
作者：
HARBRON, S
EGGELTE, HJ
RABIN, BR
机构：
[1] London Biotechnology Ltd., Department of Biochemistry and Molecular Biology, University College London, London, WC1E 6BT England, Gower Street
来源：
ANALYTICAL BIOCHEMISTRY | 1991年 / 198卷 / 01期
关键词：
D O I：
10.1016/0003-2697(91)90504-M
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Alkaline phosphatase hydrolyzes riboflavin 4′-phosphate to produce riboflavin. This is converted to riboflavin 5′-phosphate, using riboflavin kinase, which reconstitutes apoglycolate oxidase to give hologlycolate oxidase. This enzyme catalyzes the oxidation of glycolate with simultaneous production of hydrogen peroxide which is detected via the formation of a colored product through the action of peroxidase. The system allows the detection of 4 amol after a 2-h incubation. © 1991.
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页码：47 / 51
页数：5
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