CHARACTERIZATION AND USE OF A PENICILLIN ACYLASE BIOCATALYST

被引：0
|
作者：
OSPINA, SS
LOPEZMUNGUIA, A
GONZALEZ, RL
QUINTERO, R
机构：
[1] UNIV NACL AUTONOMA MEXICO, INST BIOTECNOL, APARTADO POSTAL 510-3, CUERNAVACA 62271, MEXICO
[2] UNIV NACL COLOMBIA, INST BIOTECHNOL, DEPT FARM, BOGOTA, COLOMBIA
来源：
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY | 1992年 / 53卷 / 02期
关键词：
PENICILLIN ACYLASE; CHARACTERIZATION; STABILITY; REACTORS;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A complete characterization of a penicillin acylase biocatalyst is presented, including the determination of physicochemical and kinetic parameters. Stability studies are detailed in terms of both storage temperature and pH as well as operational stability after 150 batch reactions of two hours duration each. An Arrhenius-type model was used to simulate the effect of pH on biocatalyst stability. A kinetic model is proposed to describe batch and continuous stirred tank reactors and to predict the long-term behavior of the process.
引用
收藏
页码：205 / 213
页数：9
相关论文
共 50 条
  • [31] IMMOBILIZATION OF PENICILLIN ACYLASE ON ACRYLIC CARRIERS
    BRYJAK, J
    TROCHIMCZUK, A
    NOWORYTA, A
    BIOPROCESS ENGINEERING, 1989, 4 (04): : 159 - 162
  • [32] NOVEL APPROACHES TO THE PURIFICATION OF PENICILLIN ACYLASE
    MAHAJAN, PB
    BORKAR, PS
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1984, 9 (5-6) : 421 - 437
  • [33] Expression and characterization of a thermostable penicillin G acylase from an environmental metagenomic library
    Zhang, Qian
    Xu, Hui
    Zhao, Jing
    Zeng, Runying
    BIOTECHNOLOGY LETTERS, 2014, 36 (03) : 617 - 625
  • [34] Purification and characterization of Alcaligenes faecalis penicillin G acylase expressed in Bacillus subtilis
    Zhou, Z
    Zhou, LP
    Chen, MJ
    Zhang, YL
    Li, RB
    Yang, S
    Yuan, ZY
    ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2003, 35 (05): : 416 - 422
  • [35] DEACYLATION OF BENZYLPENICILLIN BY IMMOBILIZED PENICILLIN ACYLASE
    LILLY, MD
    CARLEYSMITH, SW
    DUNNILL, P
    BIOCHIMIE, 1980, 62 (5-6) : 317 - 321
  • [36] Rhodotorula aurantiaca penicillin V acylase: Active site characterization and fluorometric studies
    Kumar, Atul
    Gowda, Nagaraj M.
    Gaikwad, Sushma
    Pundle, Archana
    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2009, 97 (02) : 109 - 116
  • [37] PENICILLIN ACYLASE EXTRACTION BY OSMOTIC SHOCK
    RODRIGUEZ, M
    GUERECA, L
    VALLE, F
    QUINTERO, R
    LOPEZMUNGUIA, A
    PROCESS BIOCHEMISTRY, 1992, 27 (04) : 217 - 223
  • [38] Efficient biocatalyst for large-scale synthesis of cephalosporins, obtained by combining immobilization and site-directed mutagenesis of penicillin acylase
    Cecchini, Davide A.
    Pavesi, Roberto
    Sanna, Sara
    Daly, Simona
    Xaiz, Roberto
    Pregnolato, Massimo
    Terreni, Marco
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 95 (06) : 1491 - 1500
  • [39] THE USE OF STABILIZED PENICILLIN ACYLASE DERIVATIVES IMPROVES THE DESIGN OF KINETICALLY CONTROLLED SYNTHESIS
    FERNANDEZLAFUENTE, R
    ROSELL, CM
    GUISAN, JM
    JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL, 1995, 101 (01) : 91 - 97
  • [40] A new support for the immobilization of penicillin acylase
    Jing, H
    Li, XF
    Evans, DG
    Duan, X
    Li, CY
    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2000, 11 (01) : 45 - 53
12345