MYOSIN LIGHT-CHAIN KINASE EXPRESSION DURING SMOOTH-MUSCLE DEVELOPMENT

被引：0
|
作者：
DALLALIBERA, LD
COLLINS, JH
机构：
[1] UNIV MARYLAND, SCH MED, DEPT BIOL CHEM, BALTIMORE, MD 21201 USA
[2] UNIV MARYLAND, MARYLAND BIOTECHNOL INST, CTR MED BIOTECHNOL, BALTIMORE, MD 21201 USA
来源：
BIOSCIENCE REPORTS | 1993年 / 13卷 / 05期
关键词：
MYOSIN LIGHT CHAIN KINASE; SMOOTH MUSCLE; DEVELOPMENT; ISOFORMS; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The expression of smooth muscle myosin light chain kinase (MLCK) was investigated during chicken gizzard development. The molecular weight and the antigenic properties of MLCK did not change during development. The use of anion exchange high performance liquid chromatography (HPLC) enabled us to distinguish between MLCKs from post-hatched and adult chickens. A partial amino acid sequence determination of ii-day-old gizzard MLCK failed to disclose differences in the primary sequences of the two proteins. The results suggest that MLCK has the same primary sequence in all stages of gizzard development, although charge variants due to post-translational modifications may exist.
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页码：289 / 295
页数：7
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