EXPRESSION OF THE HYGROMYCIN-B PHOSPHOTRANSFERASE GENE CONFERS TOLERANCE TO THE HERBICIDE GLYPHOSATE

被引：4

作者：

PENALOZAVAZQUEZ, A ^{[1
]}

OROPEZA, A ^{[1
]}

MENA, GL ^{[1
]}

BAILEY, AM ^{[1
]}

机构：

[1] IPN,CINVESTAV,UNIDAD IRAPUATO,DEPT INGN GENET PLANTAS,IRAPUATO 36500,GUANAJUATO,MEXICO

来源：

PLANT CELL REPORTS | 1995年 / 14卷 / 08期

关键词：

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Escherichia coli cells and tobacco (cv. Xanthi) plants transformed with the hygromycin B phosphotransferase gene were able to grow in culture medium containing glyphosate at 2.0 mM. The growth of tobacco calli in media containing increasing glyphosate concentrations was measured. The ID50 for glyphosate was 1.70+/-0.03 mM for hygromycin-B resistant plants, and 0.45+/-0.02 mM for control plants. Regenerated plants and progeny selected for resistance to hygromycin B were tested for glyphosate tolerance by spraying them with Faena herbicide (formulated glyphosate with surfactant) at a dose equal to 0.24 kg/ha. This was two times the dose required to kill 100 percent of the control plants. Phosphotransferase activity was measured in the extracts of the transformed leaves by the incorporation of P-32 from [gamma(32)P]ATP and it was observed that hygromycin B phosphotransferase was able to recognize the molecule of glyphosate as substrate.

引用

页码：482 / 487

页数：6

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