GLYCOSYLATION OF THE FLAGELLIN OF THE POLAR FLAGELLUM OF AZOSPIRILLUM-BRASILENSE, A GRAM-NEGATIVE NITROGEN-FIXING BACTERIUM

被引：54

作者：

MOENS, S

MICHIELS, K

VANDERLEYDEN, J

机构：

[1] CATHOLIC UNIV LEUVEN,FA JANSSENS LAB GENET,B-3001 HEVERLEE,BELGIUM

[2] CATHOLIC UNIV LEUVEN,FOOD MICROBIOL LAB,B-3001 HEVERLEE,BELGIUM

来源：

MICROBIOLOGY-UK | 1995年 / 141卷

关键词：

AZOSPIRILLUM BRASILENSE; GLYCOSYLATION; BACTERIAL GLYCOPROTEIN; POLAR FLAGELLIN; GLYCOSYLATION MUTANTS;

D O I：

10.1099/13500872-141-10-2651

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The glycosylation of the flagellin of the polar flagellum of Azospirillum brasilense Sp7 is demonstrated in several ways: (1) by a decrease in apparent M(r) after chemical deglycosylation; (2) by sugar staining after SDS-PAGE; (3) by use of a sugar-specific monoclonal antibody in immunogold labelling coupled with transmission electron microscopy. In addition, two mutant flagellins are described; they differ in glycan composition when compared with the wildtype. The glycosylation of a bacterial flagellin is unusual and has to be taken into account in the proposed model for the assembly of the flagellar filament.

引用

页码：2651 / 2657

页数：7

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