GLYCOSYLATION OF THE FLAGELLIN OF THE POLAR FLAGELLUM OF AZOSPIRILLUM-BRASILENSE, A GRAM-NEGATIVE NITROGEN-FIXING BACTERIUM

被引：54
|
作者：
MOENS, S
MICHIELS, K
VANDERLEYDEN, J
机构：
[1] CATHOLIC UNIV LEUVEN,FA JANSSENS LAB GENET,B-3001 HEVERLEE,BELGIUM
[2] CATHOLIC UNIV LEUVEN,FOOD MICROBIOL LAB,B-3001 HEVERLEE,BELGIUM
来源：
MICROBIOLOGY-UK | 1995年 / 141卷
关键词：
AZOSPIRILLUM BRASILENSE; GLYCOSYLATION; BACTERIAL GLYCOPROTEIN; POLAR FLAGELLIN; GLYCOSYLATION MUTANTS;
D O I：
10.1099/13500872-141-10-2651
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The glycosylation of the flagellin of the polar flagellum of Azospirillum brasilense Sp7 is demonstrated in several ways: (1) by a decrease in apparent M(r) after chemical deglycosylation; (2) by sugar staining after SDS-PAGE; (3) by use of a sugar-specific monoclonal antibody in immunogold labelling coupled with transmission electron microscopy. In addition, two mutant flagellins are described; they differ in glycan composition when compared with the wildtype. The glycosylation of a bacterial flagellin is unusual and has to be taken into account in the proposed model for the assembly of the flagellar filament.
引用
收藏
页码：2651 / 2657
页数：7
相关论文
共 50 条
  • [1] ASSOCIATION BETWEEN THE NITROGEN-FIXING BACTERIUM AZOSPIRILLUM-BRASILENSE AND EXCISED PLANT-ROOTS
    OHARA, GW
    DAVEY, MR
    LUCAS, JA
    ZEITSCHRIFT FUR PFLANZENPHYSIOLOGIE, 1983, 113 (01): : 1 - 13
  • [2] INSERTION MUTAGENESIS OF NITROGEN-FIXING BACTERIUM AZOSPIRILLUM BRASILENSE
    BOROVOK, IA
    MYAKINKOV, AG
    GENETIKA, 1989, 25 (01): : 36 - 48
  • [3] (Methyl)ammonium transport in the nitrogen-fixing bacterium Azospirillum brasilense
    Van Dommelen, A
    Keijers, V
    Vanderleyden, J
    de Zamaroczy, M
    JOURNAL OF BACTERIOLOGY, 1998, 180 (10) : 2652 - 2659
  • [4] HYDROGEN-UPTAKE HYDROGENASE ACTIVITY IN NITROGEN-FIXING AZOSPIRILLUM-BRASILENSE
    PEDROSA, FO
    STEPHAN, M
    DOBEREINER, J
    YATES, MG
    JOURNAL OF GENERAL MICROBIOLOGY, 1982, 128 (JAN): : 161 - 166
  • [5] AZOSPIRILLUM-BRASILENSE, A NITROGEN-FIXING BACTERIUM WHICH LACKS THE SOS ERROR-PRONE REPAIR SYSTEM
    DELGALLO, M
    GRATANI, L
    MORPURGO, G
    MUTATION RESEARCH, 1987, 178 (02): : 195 - 200
  • [6] COMPARATIVE STUDIES OF NITROGEN-FIXING BACTERIA ASSOCIATED WITH GRASSES IN ISRAEL WITH AZOSPIRILLUM-BRASILENSE
    NUR, I
    OKON, Y
    HENIS, Y
    CANADIAN JOURNAL OF MICROBIOLOGY, 1980, 26 (06) : 714 - 718
  • [7] EFFECT OF AZOSPIRILLUM-BRASILENSE ON FORMATION AND NITROGEN-FIXING ACTIVITY OF BEAN AND SOYBEAN NODULES
    ANDREEVA, IN
    MANDKHAN, K
    REDKINA, TV
    MISHUSTIN, EN
    IZMAILOV, SF
    SOVIET PLANT PHYSIOLOGY, 1991, 38 (05): : 646 - 651
  • [8] THE POLAR FLAGELLUM MEDIATES AZOSPIRILLUM-BRASILENSE ADSORPTION TO WHEAT ROOTS
    CROES, CL
    MOENS, S
    VANBASTELAERE, E
    VANDERLEYDEN, J
    MICHIELS, KW
    JOURNAL OF GENERAL MICROBIOLOGY, 1993, 139 : 2261 - 2269
  • [9] FLUORESCEIN ISOTHIOCYANATE-LABELED LECTIN ANALYSIS OF THE SURFACE OF THE NITROGEN-FIXING BACTERIUM AZOSPIRILLUM-BRASILENSE BY FLOW-CYTOMETRY
    YAGODASHAGAM, J
    BARTON, LL
    REED, WP
    CHIOVETTI, R
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (07) : 1831 - 1837
  • [10] SUPEROXIDE-DISMUTASE, CATALASE, AND PEROXIDASE IN AMMONIUM-GROWN AND NITROGEN-FIXING AZOSPIRILLUM-BRASILENSE
    CLARA, RW
    KNOWLES, R
    CANADIAN JOURNAL OF MICROBIOLOGY, 1984, 30 (10) : 1222 - 1228
12345