OBSERVATION OF THE RABBIT CORNEA AND LENS WITH A NEW REAL-TIME CONFOCAL SCANNING OPTICAL MICROSCOPE

被引：20
|
作者：
XIAO, GQ
KINO, GS
MASTERS, BR
机构：
[1] STANFORD UNIV,WW HANSEN LABS PHYS,EDWARD L GINZTON LAB,STANFORD,CA 94305
[2] GEORGIA INST TECHNOL,SCH ELECT ENGN,ATLANTA,GA 30332
来源：
SCANNING | 1990年 / 12卷 / 03期
关键词：
D O I：
10.1002/sca.4950120308
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
A new one‐sided tandem scanning microscope (OTSM) has been utilized to optically section a transparent cornea and ocular lens with submicron depth and transverse resolution. Its high resolution, image quality, and contrast, together with color capability, real‐time operation, and easy alignment, have advantages over previous confocal systems. Planes within the nuclei of surface epithelial cells, the epithelial basement membrane, nerve fibers, the interior of endothelial cells, and the transverse structure of the lens fibers are readily observed. The utility of real‐time microscopic observation of live biological structures provides a new paradigm for cell biology and diagnostic ophthalmology. Copyright © 1990 Foundation for Advances in Medicine and Science, Inc.
引用
收藏
页码：161 / 166
页数：6
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