INTERACTION OF MERCURIC IONS WITH THE BACTERIAL-GROWTH MEDIUM AND ITS EFFECTS ON ENZYMATIC REDUCTION OF MERCURY

被引：40
|
作者：
CHANG, JS
HONG, J
OGUNSEITAN, OA
OLSON, BH
机构：
[1] UNIV CALIF IRVINE,BIOCHEM ENGN PROGRAM,IRVINE,CA 92717
[2] UNIV CALIF IRVINE,DEPT ENVIRONM ANAL & DESIGN,IRVINE,CA 92717
来源：
BIOTECHNOLOGY PROGRESS | 1993年 / 9卷 / 05期
关键词：
D O I：
10.1021/bp00023a012
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The interaction of Hg2+ with components of Luria-Bertani broth (LB), used to grow the mercury-resistant strain Pseudomonas aeruginosa PU21 (Rip64), was studied using cold vapor atomic absorption spectrometry and liquid scintillation counting. The time course analysis of free Hg2+ and total Hg in LB solutions showed that 30-40 % of Hg2+ formed complexes with tryptone and yeast extract within 140 h. Media containing free or complexed Hg2+ were treated with cell-free mercuric reductase as well as with growing cells to investigate the effect of the Hg2+-LB interaction on mercury reduction. In both assays, it was found that the complexed Hg2+ Could not be reduced by mercuric reductase or P. aeruginosa PU21 (Rip64).
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页码：526 / 532
页数：7
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