POSTTRANSCRIPTIONAL REGULATION OF THE PRO-ALPHA-1(I) COLLAGEN GENE IN PRO-ALPHA-1(I)-DEFICIENT, CHEMICALLY TRANSFORMED SYRIAN-HAMSTER EMBRYO FIBROBLASTS

被引：4

作者：

SCHALK, EM ^{[1
]}

GOSIEWSKA, A ^{[1
]}

PRATHER, W ^{[1
]}

PETERKOFSKY, B ^{[1
]}

机构：

[1] NCI, BIOCHEM LAB, BLDG 37, ROOM 4C-18, BETHESDA, MD 20892 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 188卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)91124-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

4-Nitroquinoline-1-oxide-transformed Syrian hamster embryo fibroblasts (NQT-SHE) synthesize the proα2 chain but not the proα1 subunit of type I procollagen, and they contain little proα1(I) mRNA. This study shows that there was no accumulation of proα1(I) poly(A)+ mRNA in NQT-SHE fibroblasts. BHK cells, a normal established line of hamster fibroblasts that synthesized collagen at approximately the same rate as NQT-SHE fibroblasts, nevertheless produced both subunits of type I collagen and contained proα1(I) mRNA. Run-off transcription assays with isolated nuclei showed that both the proα1(I) and proα2(I) genes were transcribed at about the same rate in NQT-SHE cells as well as in the normal BHK cells. These results suggest that a post-transcriptional defect, probably resulting from transformation, prevents the accumulation of proα1(I) mRNA in NQT-SHE cells. © 1992.

引用

页码：780 / 785

页数：6

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