SITE-SPECIFIC MODIFICATION OF PRE-MESSENGER-RNA - THE 2'-HYDROXYL GROUPS AT THE SPLICE SITES

被引：427

作者：

MOORE, MJ ^{[1
]}

SHARP, PA ^{[1
]}

机构：

[1] MIT, DEPT BIOL, CAMBRIDGE, MA 02139 USA

来源：

SCIENCE | 1992年 / 256卷 / 5059期

关键词：

D O I：

10.1126/science.1589782

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A simple and efficient method for synthesizing long, site-specifically modified RNA molecules was developed whereby segments of RNA were joined with the use of bacteriophage T4 DNA ligase. A single hydrogen or O-methyl group was substituted for the 2'-hydroxyl group at either splice site of a nuclear pre-messenger RNA substrate. Splicing of the modified pre-messenger RNA's in vitro revealed that, although a 2'-hydroxyl is not absolutely required at either splice site, the 2'-hydroxyl at the 3' splice site is important for the second step of splicing. These results are compared to previous studies of analogous 2'-hydroxyl groups in the self-splicing Tetrahymena group I intron.

引用

页码：992 / 997

页数：6

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