DIFFERENTIATION OF VENEZUELAN EQUINE ENCEPHALOMYELITIS COMPLEX VIRUSES USING MONOCLONAL-ANTIBODIES AND TIME-RESOLVED FLUOROIMMUNOASSAY

被引：0
|
作者：
GAIDAMOVICH, SY
POMELOVA, VG
LAVROVA, NA
MELNIKOVA, EE
SOKOLOVA, MV
KHARITONENKOV, IG
ZLOBIN, VN
机构：
来源：
VOPROSY VIRUSOLOGII | 1991年 / 36卷 / 03期
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中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Potentialities of differentiation between Venezuelan equine encephalomyelitis (VEE) complex viruses by time-resolved fluoroimmunoassay and enzyme immunoassay were studied. For this, 4 test systems were used based on different combinations of native and labeled polyclonal antibodies to VEE virus, strain Trinidad, and monoclonal (MCA) antibody MAK 14-7 to protein EL of this virus. The maximal sensitivity and specificity was achieved in the test system formed from native MCA MAK 14-7 for sensitization of the solid phase and labeled polyclonal immunoglobulins for demonstration of the test results. This combination of antibodies allowed to differentiate the epidemic variant of VEF/Trinidad (IA) from epizootic variants of Mucambo (III), Pixuna (IV) and attenuated strain No. 230.
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页码：226 / 229
页数：4
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