CRYSTAL-STRUCTURE OF TRICHOSANTHIN-NADPH COMPLEX AT 1.7 ANGSTROM RESOLUTION REVEALS ACTIVE-SITE ARCHITECTURE

被引：47

作者：

XIONG, JP ^{[1
]}

XIA, ZX ^{[1
]}

WANG, Y ^{[1
]}

机构：

[1] CHINESE ACAD SCI,SHANGHAI INST ORGAN CHEM,SHANGHAI 200032,PEOPLES R CHINA

来源：

NATURE STRUCTURAL BIOLOGY | 1994年 / 1卷 / 10期

关键词：

D O I：

10.1038/nsb1094-695

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe here the crystal structure of the trichosanthin-NADPH complex determined at a resolution of 1.7 Angstrom. The adenine base stacks between Tyr 70 and Tyr 111. Aug 163, Glu 160 and Tyr 70 form hydrogen bonds to N(3), O(3') and, through a water molecule, to N(9) of adenosine, respectively. This is the first high resolution structure of a complex between a ribosome-inactivating protein and a substrate analogue, in which the electron density of the IV-glycosidic bond is well defined and the preassociated water, thought to be responsible for hydrolyzing the N-C bond, is also explicitly elucidated.

引用

页码：695 / 700

页数：6

共 50 条

[31] TOWARDS STRUCTURE-BASED DRUG DESIGN - CRYSTAL-STRUCTURE OF A MULTISUBSTRATE ADDUCT COMPLEX OF GLYCINAMIDE RIBONUCLEOTIDE TRANSFORMYLASE AT 1.96 ANGSTROM RESOLUTION
KLEIN, C
CHEN, P
AREVALO, JH
STURA, EA
MAROLEWSKI, A
WARREN, MS
BENKOVIC, SJ
WILSON, IA
JOURNAL OF MOLECULAR BIOLOGY, 1995, 249 (01) : 153 - 175
[32] CRYSTAL-STRUCTURE DETERMINATION AT 2.3-ANGSTROM OF RECOMBINANT HUMAN DIHYDROFOLATE-REDUCTASE TERNARY COMPLEX WITH NADPH AND METHOTREXATE-GAMMA-TETRAZOLE
CODY, V
LUFT, JR
CISZAK, E
KALMAN, TI
FREISHEIM, JH
ANTI-CANCER DRUG DESIGN, 1992, 7 (06): : 483 - 491
[33] CRYSTAL-STRUCTURE OF BOVINE BETA-TRYPSIN AT 1.5-A RESOLUTION IN A CRYSTAL FORM WITH LOW-MOLECULAR PACKING DENSITY - ACTIVE-SITE GEOMETRY, ION-PAIRS AND SOLVENT STRUCTURE
BARTUNIK, HD
SUMMERS, LJ
BARTSCH, HH
JOURNAL OF MOLECULAR BIOLOGY, 1989, 210 (04) : 813 - 828
[34] Crystal structure of the wild-type human procathepsin B at 2.5 angstrom resolution reveals the native active site of a papain-like cysteine protease zymogen
Podobnik, M
Kuhelj, R
Turk, V
Turk, D
JOURNAL OF MOLECULAR BIOLOGY, 1997, 271 (05) : 774 - 788
[35] CRYSTAL-STRUCTURE OF ESCHERICHIA-COLI RNASE HI IN COMPLEX WITH MG2+ AT 2.8 ANGSTROM RESOLUTION - PROOF FOR A SINGLE MG2+-BINDING SITE
KATAYANAGI, K
OKUMURA, M
MORIKAWA, K
PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1993, 17 (04): : 337 - 346
[36] FLUORIDE INHIBITION OF YEAST ENOLASE - CRYSTAL-STRUCTURE OF THE ENOLASE-MG2+-F--PI COMPLEX AT 2.6 ANGSTROM RESOLUTION
LEBIODA, L
ZHANG, E
LEWINSKI, K
BREWER, JM
PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1993, 16 (03): : 219 - 225
[37] MECHANISM OF THE REACTION CATALYZED BY MANDELATE RACEMASE .2. CRYSTAL-STRUCTURE OF MANDELATE RACEMASE AT 2.5-A RESOLUTION - IDENTIFICATION OF THE ACTIVE-SITE AND POSSIBLE CATALYTIC RESIDUES
NEIDHART, DJ
HOWELL, PL
PETSKO, GA
POWERS, VM
LI, RS
KENYON, GL
GERLT, JA
BIOCHEMISTRY, 1991, 30 (38) : 9264 - 9273
[38] The 1.7 Å crystal structure of the C5a peptidase from Streptococcus agalactiae (ScpB) reveals an active site competent for catalysis
Cullen, Ruth
Tecza, Malgorzata
Miclot, Tom
Behan, Senan
Jain, Monica
Avink, Marjet Klein
Cooney, Jakki C.
Kagawa, Todd F.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 2024, 92 (03) : 427 - 431
[39] Crystal structure of the papain-like protease of MERS coronavirus reveals unusual, potentially druggable active-site features
Lei, Jian
Mesters, Jeroen R.
Drosten, Christian
Anemueller, Stefan
Ma, Qingjun
Hilgenfeld, Rolf
ANTIVIRAL RESEARCH, 2014, 109 : 72 - 82
[40] REFINED CRYSTAL-STRUCTURE OF BOVINE BETA-TRYPSIN AT 1.8 A RESOLUTION .2. CRYSTALLOGRAPHIC REFINEMENT, CALCIUM-BINDING SITE, BENZAMIDINE BINDING-SITE AND ACTIVE-SITE AT PH 7.0
BODE, W
SCHWAGER, P
JOURNAL OF MOLECULAR BIOLOGY, 1975, 98 (04) : 693 - 717

← 1 2 3 4 5 →