MEMBRANE DEPOLARIZATION INHIBITS THROMBIN-INDUCED CALCIUM INFLUX AND AGGREGATION IN HUMAN PLATELETS

被引：11
|
作者：
KOVACS, T [1 ]
TORDAI, A [1 ]
SZASZ, I [1 ]
SARKADI, B [1 ]
GARDOS, G [1 ]
机构：
[1] NATL INST HAEMATOL & BLOOD TRANSFUS,DAROCZI U 24,H-1113 BUDAPEST,HUNGARY
来源：
FEBS LETTERS | 1990年 / 266卷 / 1-2期
关键词：
Aggregation; Calcium flux; Human platelet; Membrane potential; Receptor-operated calcium channel; Thrombin;
D O I：
10.1016/0014-5793(90)81532-S
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The relationship between thrombin-evoked changes in intracellular calcium concentration ([Ca2+]i) and aggregation was examined in Indo-1-loaded human platelets. The stimulus-induced intracellular calcium release and external calcium influx, as well as platelet aggregation, were studied in the same cell preparation. A close correlation between the sustained high [Ca2+]i level, depending on calcium entry, and the aggregation response was found. Gramicidin, at a concentration high enough to induce membrane depolarization, strongly inhibited the calcium influx and aggregation, but did not influence the thrombin-induced intracellular calcium release. We conclude that calcium influx through depolarization-inhibited calcium channels is a prerequisite of thrombin-induced platelet aggregation. © 1990.
引用
收藏
页码：171 / 174
页数：4
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