REGULATION OF CGMP PRODUCTION BY INTRACELLULAR ALKALINIZATION IN CULTURED RAT INNER MEDULLARY COLLECTING DUCT CELLS

被引：3
|
作者：
SISKIND, MS
ALEXANDER, EA
SCHWARTZ, JH
机构：
[1] Thorndike Memorial Laboratory, Renal Section, Boston City Hospital, Boston, MA 02118
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 170卷 / 02期
关键词：
D O I：
10.1016/0006-291X(90)92170-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We evaluated the relationship between cell pH and cGMP production in cultured rat renal inner medullary collecting duct cells. The cGMP level, 21±6, was not different in control vs. alkalinized cells, 49±17 fmol/mg protein (p>0.5). 10-11M atrial natriureitic peptide (ANF) enhanced cGMP production in alkalinized cells, 426±34 vs. 141±9{black star}. Conversely, alkalinization inhibited 10-4M nitroprusside (SNP) induced cGMP formation, 29±9 vs. 332±67{black star}. Phosphodiesterase inhibition abolished the difference in cGMP production by ANF but did not reverse the inhibitory effect of alkalinization on SNP induced cGMP production. In rat renal inner medullary collecting duct cells, cellular alkalinization plays a significant role in the regulation of guanylate cyclase mediated cGMP production. {black star}=p<0.05. © 1990.
引用
收藏
页码：860 / 866
页数：7
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