PREPARATIVE CAPILLARY ELECTROPHORESIS BASED ON ADSORPTION OF THE SOLUTES (PROTEINS) ONTO A MOVING BLOTTING MEMBRANE AS THEY MIGRATE OUT OF THE CAPILLARY

被引：37
|
作者：
ERIKSSON, KO [1 ]
PALM, A [1 ]
HJERTEN, S [1 ]
机构：
[1] UNIV UPPSALA, CTR BIOMED, DEPT BIOCHEM, POB 576, S-75123 UPPSALA, SWEDEN
来源：
ANALYTICAL BIOCHEMISTRY | 1992年 / 201卷 / 02期
关键词：
D O I：
10.1016/0003-2697(92)90330-A
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A micropreparative capillary electrophoresis apparatus equipped with a new type of fraction collection device is described: solutes, such as proteins, are adsorbed onto a moving blotting membrane (for instance a polyvinylidene difluoride membrane) as they migrate electrophoretically out of the capillary. The adsorbed proteins are visualized by conventional protein staining methods or be fluorescent labeling. Specific identification of separated components by an immunological technique is demonstrated. The method also offers the potential to analyze proteins and peptides collected on the membrane by gas phase sequencing and mass spectrometry. © 1992.
引用
收藏
页码：211 / 215
页数：5
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