MEASUREMENT OF ALLERGEN-SPECIFIC IGD AND CORRELATION WITH ALLERGEN-SPECIFIC IGE

被引:14
|
作者
ZHANG, M [1 ]
NIEHUS, J [1 ]
BRUNNEE, T [1 ]
KLEINETEBBE, J [1 ]
OCONNOR, A [1 ]
KUNKEL, G [1 ]
机构
[1] FREE UNIV BERLIN,DEPT CLIN IMMUNOL & ASTHMA-OPD,D-13353 BERLIN,GERMANY
关键词
D O I
10.1111/j.1365-3083.1994.tb03496.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
A new method for the measurement of allergen-specific IgD (as-IgD) was developed by modifying the ImmunoCAP assay (Pharmacia), and amplification of the signal with a goat anti-human/rabbit anti-goat detection system. The assay was sensitive enough to measure as-IgD in serum samples. The specificity of the assay was examined using inhibition tests with excess corresponding and noncorresponding allergens. For the different allergens inhibition rates between 56% (house dust mite) and 88% (cat) could be achieved. Non-corresponding allergens did not inhibit the as-IgD binding. Total IgE and allergen-specific IgE (as-IgE) was measured using the ImmunoCAP system. Total IgD was measured using a sandwich ELISA. As-IgD was measured in serum samples from 51 atopic and 23 nonatopic subjects, and the correlation with as-IgE was examined. As-IgD was detected in both atopics and non-atopics but at higher levels in atopics. As-IgD against birch pollen and timothy pollen allergen was found to be increased in atopics with IgE directed against these allergens compared to atopics without IgE against these allergens (P < 0.02 and P < 0.03). As-IgD against birch pollen allergen was higher in atopics with IgE specific to this allergen than in non-atopics (P < 0.02). In contrast to total IgE and total IgD, significant correlations were observed between as-IgD and as-IgE against timothy pollen (r = 0.34, P < 0.04), birch pollen (r = 0.38, P < 0.05) and cat dander allergen (r = 0.52, P < 0.01). The observed correlations between as-IgD and IgE suggest that IgD and IgE may be similarly regulated, and thus the measurement of as-IgD may give further insight into the regulation of IgE.
引用
收藏
页码:502 / 508
页数:7
相关论文
共 50 条
  • [1] Evolution of the Identification of Allergen-Specific IgE
    Reisman, Robert E.
    [J]. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2009, 123 (02) : S1 - S1
  • [2] Allergen-specific IgE in nonatopic dogs
    Layne, Elizabeth A.
    DeBoer, Douglas J.
    [J]. VETERINARY DERMATOLOGY, 2019, 30 (01) : 78 - 79
  • [3] Induction and regulation of allergen-specific IgE
    Jeurink, PV
    Savelkoul, HFJ
    [J]. ALLERGY MATTERS: NEW APPROACHES TO ALLERGY PREVENTION AND MANAGEMENT, 2006, 10 : 13 - +
  • [4] Influence of Chemotherapy on Allergen-Specific IgE
    Whiteside, Sarah
    Markova, Marketa
    Chin, Alex
    Lam, Cynthia
    Dharmani-Khan, Poonam
    Modi, Monica
    Khan, Faisal
    Storek, Jan
    [J]. INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 2018, 177 (02) : 145 - 152
  • [5] ALLERGEN-SPECIFIC ELISA FOR HORSE IGE
    SUTER, M
    FEY, H
    [J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 1983, 4 (5-6) : 555 - 564
  • [6] TOTAL IGE AND ALLERGEN-SPECIFIC IGE ASSAYS
    WOODMANSEY, N
    [J]. ANNALS OF CLINICAL BIOCHEMISTRY, 1988, 25 : 196 - 196
  • [7] TOTAL IGE AND ALLERGEN-SPECIFIC IGE ASSAYS
    MARINKOVICH, VA
    [J]. ANNALS OF CLINICAL BIOCHEMISTRY, 1988, 25 : 195 - 196
  • [8] Allergen-specific IgG antibody levels modify the relationship between allergen-specific IgE and wheezing in childhood
    Custovic, Adnan
    Soderstrom, Lars
    Ahlstedt, Staffan
    Sly, Peter D.
    Simpson, Angela
    Holt, Patrick G.
    [J]. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2011, 127 (06) : 1480 - 1485
  • [9] Correlation dependence between allergen-specific IgE and scarification tests
    Irsalieva, F.
    [J]. ALLERGY, 2008, 63 : 201 - 201
  • [10] Streptavidin ImmunoCAP: a valuable tool for measurement of allergen-specific IgE
    Sander, I
    Gruehn, S
    Bruening, T
    Raulf-Heimsoth, M
    [J]. ALLERGY, 2002, 57 : 221 - 221