GLUCOSE BIOSENSOR BASED ON THE INCORPORATION OF MELDOLA BLUE AND GLUCOSE-OXIDASE WITHIN CARBON-PASTE

被引：32
|
作者：
KULYS, J [1 ]
HANSEN, HE [1 ]
BUCHRASMUSSEN, T [1 ]
WANG, J [1 ]
OZSOZ, M [1 ]
机构：
[1] NEW MEXICO STATE UNIV,DEPT CHEM & BIOCHEM,LAS CRUCES,NM 88003
来源：
ANALYTICA CHIMICA ACTA | 1994年 / 288卷 / 03期
关键词：
BIOSENSORS; GLUCOSE; GLUCOSE OXIDASE; MELDOLA BLUE MEDIATOR;
D O I：
10.1016/0003-2670(93)E0610-J
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The phenoxazine compound Meldola Blue (MB) is shown to efficiently mediate the electron transfer from reduced glucose oxidase to a conventional carbon paste electrode. The mediation process is exploited for developing an amperometric biosensor for glucose, which yields a linear response to 0-25 mM glucose at an operating potential of 50 mV (vs. SCE), where interfering reactions do not occur. Experimental variables such as enzyme loading or operating potential are explored. A stable response is observed over several months.
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页码：193 / 196
页数：4
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