HYPOTONIC TREATMENT LEADS TO CHROMOSOMAL-ABERRATIONS BUT NOT TO SISTER-CHROMATID EXCHANGES IN HUMAN-LYMPHOCYTES

被引：11
|
作者：
KALWEIT, S
NOWAK, C
OBE, G
机构：
[1] UNIV ESSEN GESAMTHSCH,FACHBEREICH 9,DEPT GENET,POB 103764,W-4300 ESSEN 1,GERMANY
[2] FREE UNIV BERLIN,INST GENET,W-1000 BERLIN 33,GERMANY
来源：
MUTATION RESEARCH | 1990年 / 245卷 / 01期
关键词：
Chromosome aberration; Human lymphocytes; Hypotonic treatment;
D O I：
10.1016/0165-7992(90)90017-E
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Human peripheral lymphocytes were isolated from whole blood and exposed to culture medium of reduced osmolality. This hypotonic treatment led to a significant increase in the frequencies of chromosomal aberrations when the osmolality was reduced to 60 mOsm/kg H20 and below. Maximum damage occurred when the hypotonic treatment was done 27 or 30 h after starting the cultures. We also looked for the induction of sister-chromatid exchanges (SCE) by hypotonic culture conditions, but the SCE frequencies were not influenced. © 1990.
引用
收藏
页码：5 / 9
页数：5
相关论文
共 50 条
  • [1] EXPRESSION PATTERN OF CHROMOSOMAL-ABERRATIONS AND SISTER-CHROMATID EXCHANGES IN HUMAN-LYMPHOCYTES
    SBRANA, I
    SBRANA, M
    CECCHERINI, I
    LOPRIENO, N
    [J]. MUTATION RESEARCH, 1988, 203 (03): : 222 - 222
  • [2] THE EFFECTS OF METRONIDAZOLE ON THE FREQUENCY OF SISTER-CHROMATID EXCHANGES AND CHROMOSOMAL-ABERRATIONS IN HUMAN-LYMPHOCYTES INVITRO AND INVIVO
    LAMBERT, B
    LINDBLAD, A
    [J]. MUTATION RESEARCH, 1980, 74 (03): : 230 - 230
  • [3] INDUCTION OF CHROMOSOMAL-ABERRATIONS AND SISTER-CHROMATID EXCHANGES BY AIRBORNE PARTICULATE MATTER IN HUMAN-LYMPHOCYTES INVITRO
    HADNAGY, W
    SEEMAYER, NH
    [J]. MUTATION RESEARCH, 1987, 181 (02): : 319 - 319
  • [4] EFFECT OF PERMETHRIN ON THE INDUCTION OF CHROMOSOMAL-ABERRATIONS, SISTER-CHROMATID EXCHANGES AND MICRONUCLEI IN CULTURED HUMAN-LYMPHOCYTES
    BARRUECO, C
    HERRERA, A
    CABALLO, C
    SANTAMARIA, A
    SANZ, F
    DELAPENA, E
    [J]. MUTATION RESEARCH, 1992, 271 (02): : 137 - 137
  • [5] CHROMOSOMAL-ABERRATIONS AND SISTER-CHROMATID EXCHANGES IN SWINE
    RUBES, J
    [J]. MUTATION RESEARCH, 1987, 191 (02): : 105 - 109
  • [6] NO LIQUID HOLDING RECOVERY FOR CHROMOSOMAL-ABERRATIONS OR SISTER-CHROMATID EXCHANGES IN IRRADIATED G1 HUMAN-LYMPHOCYTES
    KALE, R
    BENDER, MA
    [J]. MUTATION RESEARCH, 1983, 122 (01): : 53 - 58
  • [7] INDUCTION OF CHROMOSOMAL-ABERRATIONS AND SISTER CHROMATID EXCHANGES BY PROPANE SULTONE IN HUMAN-LYMPHOCYTES INVITRO
    KAUL, BL
    [J]. TOXICOLOGY LETTERS, 1985, 28 (2-3) : 139 - 142
  • [8] CHROMOSOMAL-ABERRATIONS AND SISTER-CHROMATID EXCHANGES IN TOOL AND DIE WORKERS
    GARRY, VF
    NELSON, RL
    WHORTON, EP
    WIENCKE, JK
    [J]. MUTATION RESEARCH, 1989, 225 (1-2): : 1 - 9
  • [9] SISTER-CHROMATID EXCHANGES AND CHROMOSOMAL-ABERRATIONS IN A POPULATION EXPOSED TO PESTICIDES
    DULOUT, FN
    PASTORI, MC
    OLIVERO, OA
    CID, MG
    LORIA, D
    MATOS, E
    SOBEL, N
    DEBUJAN, EC
    ALBIANO, N
    [J]. MUTATION RESEARCH, 1985, 143 (04): : 237 - 244
  • [10] CHROMOSOMAL-ABERRATIONS AND SISTER-CHROMATID EXCHANGES IN WORKERS EXPOSED TO STYRENE
    CAMURRI, L
    CODELUPPI, S
    PEDRONI, C
    SCARDUELLI, L
    [J]. MUTATION RESEARCH, 1983, 119 (3-4): : 361 - 369
12345