STREPTOMYCES-LIVIDANS GLYCOSYLATES AN EXOGLUCANASE (CEX) FROM CELLULOMONAS-FIMI

被引：21
|
作者：
MACLEOD, AM [1 ]
GILKES, NR [1 ]
ESCOTECARLSON, L [1 ]
WARREN, RAJ [1 ]
KILBURN, DG [1 ]
MILLER, RC [1 ]
机构：
[1] UNIV BRITISH COLUMBIA,DEPT MICROBIOL,300-6174 UNIV BLVD,VANCOUVER V6T 1Z3,BC,CANADA
来源：
GENE | 1992年 / 121卷 / 01期
基金：
加拿大自然科学与工程研究理事会;
关键词：
CELLULASE; SECRETION; RECOMBINANT DNA; HETEROLOGOUS EXPRESSION; GLUCANASE;
D O I：
10.1016/0378-1119(92)90173-M
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Exoglucanase Cex from Cellulomonas fimi is a glycoprotein [Langsford et al., J. Gen. Microbiol. 130 (1984) 1367-1376]. Cex produced by Streptomyces lividans from the cloned cex gene is also glycosylated. The extent and nature of glycosylation are similar for Cex from both organisms. The glycosylation affords protection against proteolysis for the enzymes from both organisms when they are bound to cellulose, but not in solution. The ability to glycosylate cloned gene products enhances the utility of Streptomyces as a host for the production of heterologous polypeptides.
引用
收藏
页码：143 / 147
页数：5
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