LARGE-SCALE, LIQUID-PHASE SYNTHESIS OF OLIGONUCLEOTIDES BY THE PHOSPHORAMIDITE APPROACH

被引：65
|
作者：
BONORA, GM
BIANCOTTO, G
MAFFINI, M
SCREMIN, CL
机构：
[1] Department of Organic Chemistry, Biopolymer Research Center CNR, University of Padova
来源：
NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 05期
关键词：
D O I：
10.1093/nar/21.5.1213
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new method for the liquid phase synthesis of oligonucleotides is described which makes use of polyethylene glycol (PEG) as soluble support and phosphoramidite derivatives as synthons. The new synthetic protocol was applied to a quite large scale production (about 100 mumoles) of such compounds up to the 20mer level. This solution method, called HELP High Efficiency Liquid Phase) Plus, appears effective in terms of speed and co ling yield and can be evaluated for the production of large amount of oligonucleotides.
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页码：1213 / 1217
页数：5
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