Quantification of ecdysteroids by immunoassay: Comparison of enzyme immunoassay and radioimmunoassay

被引：0
|
作者：
Pascual, N
Belles, X
Delbecque, JP
Hua, YJ
Koolman, J
机构：
[1] CSIC,CTR INVEST & DESARROLLO,DEPT AGROBIOL,E-08034 BARCELONA,SPAIN
[2] UNIV BOURGOGNE,ZOOL LAB,CNRS URA 674,F-21000 DIJON,FRANCE
[3] UNIV MARBURG,INST PHYSIOL CHEM,D-35033 MARBURG,GERMANY
来源：
ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES | 1995年 / 50卷 / 11-12期
关键词：
ecdysone; ecdysteroid; enzyme immunoassay; radioimmunoassay;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The performance of enzyme immunoassay (EIA) and radioimmunoassay (RTA) in the quantitative analysis of ecdysteroids was compared. The EIA was found to be at least equivalent to the RIA with respect to analytical range and sensitivity and to be more comfortable with respect to safety and time saving. When biological samples were analyzed by both assays a good correlation (r = 0.83) was found. Since the EIA has certain advantages over the RIA, we now recommend the use of the former assay for the quantification of ecdysteroids.
引用
收藏
页码：862 / 867
页数：6
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