INFLUENCE OF NUCLEAR BACKGROUND ON TRANSCRIPTION OF A CHIMERIC GENE (ORF256) AND COXI IN FERTILE AND CYTOPLASMIC MALE-STERILE WHEATS

被引:19
|
作者
SONG, JS [1 ]
HEDGCOTH, C [1 ]
机构
[1] KANSAS STATE UNIV,DEPT BIOCHEM,MANHATTAN,KS 66506
关键词
CYTOPLASMIC MALE STERILITY; WHEAT; MITOCHONDRIAL DNA; MITOCHONDRIAL RNA; COXI;
D O I
10.1139/g94-028
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Crosses between Triticum timopheevi, as maternal donor, and T. aestivum can lead to cytoplasmic male sterile (cms) plants. The T. timopheevi derived mitochondrial DNA from parental, cms, and fertility-restored lines differs from that of I aestivum derived mtDNA in the coxI gene region. Our previous results for ems lines showed that there is an open reading frame, orf256, upstream from coxI in T. timopheevi derived mtDNA that is not present in T. aestivum DNA. The 5' flanking region and the first 33 nucleotides of the coding region of orf256 are identical to the corresponding region of T. aestivum coxI, whereas the rest of orf256, including the 3' flank, is not related to coxI. Also, the organization of orf256 and coxI on a HindIII fragment from T. timopheevi derived mtDNA are identical in T. timopheevi, ems, and fertility-restored lines. We now report that the DNA sequence of orf256 is identical in T. timopheevi, ems, and fertility-restored lines. Major transcripts in ems and fertility-restored lines encode both orf256 and coxI with 5' termini like coxI mRNA of T. aestivum, whereas parental mitochondria from T. timopheevi have major transcripts with 5' termini within the orf256 coding region. Mitochondria from ems and fertility-restored lines have the potential to produce a protein that would not be present in parental T. timopheevi or in T. aestivum.
引用
收藏
页码:203 / 209
页数:7
相关论文
共 23 条
  • [21] Activation of Mitochondrial orf355 Gene Expression by a Nuclear-Encoded DREB Transcription Factor Causes Cytoplasmic Male Sterility in Maize
    Xiao, Senlin
    Zang, Jie
    Pei, Yuanrong
    Liu, Jie
    Liu, Juan
    Song, Wei
    Shi, Zi
    Su, Aiguo
    Zhao, Jiuran
    Chen, Huabang
    MOLECULAR PLANT, 2020, 13 (09) : 1270 - 1283
  • [22] Alterations of RNA editing for the mitochondrial ATP9 gene in a new orf220-type cytoplasmic male-sterile line of stem mustard (Brassica juncea var. tumida)
    Yang, Jing-Hua
    Zhang, Ming-Fang
    Yu, Jing-Quan
    JOURNAL OF INTEGRATIVE PLANT BIOLOGY, 2007, 49 (05) : 672 - 677
  • [23] CHARACTERIZATION OF THE MITOCHONDRIAL ORFB GENE AND ITS DERIVATIVE, ORF224, A CHIMERIC OPEN READING FRAME SPECIFIC TO ONE MITOCHONDRIAL GENOME OF THE POLIMA MALE-STERILE CYTOPLASM IN RAPESEED (BRASSICA-NAPUS L)
    HANDA, H
    GUALBERTO, JM
    GRIENENBERGER, JM
    CURRENT GENETICS, 1995, 28 (06) : 546 - 552