IN-VITRO PROPAGATION OF GENTIANA-KURROO - AN INDIGENOUS THREATENED PLANT OF MEDICINAL IMPORTANCE

被引:61
|
作者
SHARMA, N
CHANDEL, KPS
PAUL, A
机构
[1] National Plant Tissue Culture Repository, NBPGR, New Delhi
关键词
AXILLARY SHOOT SPROUTING; CLONAL PROPAGATION;
D O I
10.1007/BF00029722
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Shoot multiplication of Gentiana kurroo Royle, a threatened medicinal plant species, was achieved in vitro using shoot tips and nodal segments as explants. Fifteen-fold shoot multiplication occurred every 6 weeks on Murashige and Skoog's medium (MS) containing 8.9 muM benzyladenine and 1.1 muM 1-naphthaleneacetic acid. Rooting was accomplished successfully in excised shoots grown on MS basal medium containing 6% sucrose.
引用
收藏
页码:307 / 309
页数:3
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