A NUCLEAR PHOTOSYNTHETIC ELECTRON-TRANSPORT MUTANT OF ARABIDOPSIS-THALIANA WITH ALTERED EXPRESSION OF THE CHLOROPLAST, PETA GENE

被引:18
|
作者
DINKINS, RD [1 ]
BANDARANAYAKE, H [1 ]
GREEN, BR [1 ]
GRIFFITHS, AJF [1 ]
机构
[1] UNIV BRITISH COLUMBIA,DEPT BOT,VANCOUVER V6T 1Z4,BC,CANADA
关键词
ARABIDOPSIS THALIANA; HIGH CHLOROPHYLL FLUORESCENCE; CYTOCHROME F; PETA;
D O I
10.1007/BF00357174
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The nuclear photosynthetic mutant, hcf2, of Arabidopsis thaliana was isolated by screening M(2) seedlings for abnormally-high chlorophyll fluorescence (hcf), indicative of a block in photosynthetic electron transport. Fluorescence induction kinetics, photosynthetic electron transport activity assays and immunoblotting revealed that all the complexes involved in photosynthetic electron transport were affected to some extent. The most striking effect of the mutation was on the relative steady state levels of the petA transcript (encoding the apoprotein of cytochrome f) which were more than five-times higher in the mutant plants than in their wild-type siblings.
引用
收藏
页码:282 / 288
页数:7
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