GREATER SUSCEPTIBILITY TO MUTATIONS IN LAGGING-STRAND OF DNA-REPLICATION IN ESCHERICHIA-COLI THAN IN LEADING-STRAND

被引：110
|
作者：
VEAUTE, X
FUCHS, RPP
机构：
[1] Unite Propre de Recherche Cancerogenese et Mutagenese Moleculaire et Structurale, Institut de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, 67084 Strasbourg, 15, rue Descartes
来源：
SCIENCE | 1993年 / 261卷 / 5121期
关键词：
D O I：
10.1126/science.8342022
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Models of DNA replication in Escherichia coli involve an asymmetric DNA polymerase complex that replicates concurrently the leading and the lagging strands of double-stranded DNA. The effect of asymmetry on mutagenesis was tested with pairs of plasmids containing the unidirectional ColE1 origin of replication and a single lesion located in the leading or lagging strand. The lesion used was the covalent adduct that the chemical carcinogen N-2-acetylaminofluorene (AAF) forms with the C-8 position of guanine. Whether SOS was induced or not, mutations arose at about a 20-fold higher frequency when the AAF adduct was located in the lagging strand than when in the leading strand.
引用
收藏
页码：598 / 600
页数：3
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