Determination of Total Flavonoids in Acanthopanax senticosus(Rupr. et Maxim.) Harms by Enzymolysis and Its Technological Optimization

被引:0
|
作者
Li Xinzhi [1 ]
Wang Yudan [1 ]
Wang Meiliang [1 ]
Liu Tianyang [2 ]
Gao Ruihua [1 ]
Qiu Youwen [1 ]
Zhang Shunjie [3 ]
机构
[1] College of Life Sciences, Northeast Agricultural University
[2] College of Horticulture and Landscape Architecture, Northeast Agricultural University
[3] Mudanjiang Branch of Heilongjiang Forestry Science College
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中图分类号
R284.1 [化学分析与鉴定];
学科分类号
1008 ;
摘要
In order to optimize the ultrasonic extraction technique for the total flavonoid of leaf yellows plus, the contents of 21 leaf yellows plus total flavonoid from four regions in Heilongjiang Province were comparatively analyzed. The ultrasonic extraction technology was optimized by Box-Behnken response surface method, and the total flavonoid content of 21 kinds of Acanthopanax senticosus(Rupr. et Maxim.) Harms from different producing areas were analyzed by cluster analysis. The optimal process conditions were determined as ultrasonic time 30 min, solid-liquid ratio 1 : 12 and ultrasonic power 250 W, and the average yield of the total flavonoid was 1.453 mg·g-1. By optimizing the ultrasonic-assisted extraction method, the total flavonoid content from different producing areas was compared in the experiment, which provided certain data support for the optimization of the extraction process in the future and laid a certain theoretical foundation for the quality analysis of Chinese medicinal materials.
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页码:53 / 60
页数:8
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