Determination of Oleuropein in Cosmetics by Ultra Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry

An ultrahigh performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLCMS/MS) was established to quickly and accurately determine the content of oleuropein in cosmetics. The samples were extracted with methanol-aqueous solution, and the mobile phase with methanol-formic acid solution(0.1 mol/L)= 40∶60 was separated by Agilent ZORBAX Eclipse Plus C18(2.1 mm ×50 mm × 1.8 μm-Micron)column temperature 30 ℃, flow rate 0.3 mL/min. The MS end was detected by electrospray negative mode ionization(ESI-) and multiple reaction monitoring(MRM) mode. The results show a good linear relationship in the range of 0.002～5 mg/L, with a correlation coefficient R2of 0.999,5. Method recovery range from 84.2%～107.6% and the relative standard deviation RSD is 5.8%. The detection time is 5 min, the detection limit is 0.000,6 mg/L, and the limit of quantification is 0.002 mg/L. This method has the advantages of convenient operation, low quantification limit, high precision and good repeatability, and is suitable for measuring the content of oleuropein in many kinds of cosmetics.