Lutein-stevioside nanoparticle attenuates H2O2-induced oxidative damage in ARPE cells

In order to improve the bioavailability of lutein(LUT),a novel lutein-stevio side nanoparticle(LUT-STE) were prepared previously,but the information about LUT-STE on protecting of eye health was limited.This study investigated the effect of LUT-STE on antioxidant activity of H2O2-induced human retinal pigment epithelial(ARPE) cells.LUT and LUT-STE(final concentration of 5 μg/mL) significantly enhanced cell viability from(74.84±5.10)% to(81.92±10.01)%(LUT) and(89.33±4.34)%(LUT-STE),and inhibited the cell apoptosis(P <0.05).After pretreatment with LUT-STE in ARPE cells,the levels of superoxide dismutase(SOD),catalase(CAT) and glutathion peroxidase(GSH-Px) in ARPE cells were significantly increased(P<0.05),the contents of reactive oxygen species(ROS) and malondialdehyde(MDA) were decreased.In addition,the vascular endothelial growth factor(VEGF) levels were inhibited by 13.61% and 17.39%,respectively,pretreatment with LUT and LUT-STE.Western blotting results showed that the pretreatment with LUT-STE inhibited the expression of caspase-9 and caspase-3 and up-regulated Bcl-2/Bax pathway to inhibit H2O2-induced apoptosis.In summary,the novel delivery LUT-STE had more pronounced inhibitory effect on H2O2-induced damage in human ARPE cells.