Thermodynamics and kinetics of lysozyme adsorption onto two kinds of weak cation exchangers

被引：0
|
作者：
Yong-Mei Cheng
Xiong-Hua Jin
Dong Gao
Hai-Feng Xia
Jing-Hua Chen
机构：
[1] Jiangnan University,School of Pharmaceutical Science
[2] Jiangnan University,National Engineering Laboratory for Cereal Fermentation Technology
[3] Northwest University,Key Laboratory of Synthetic and Natural Functional Molecular Chemistry of Ministry of Education, Institute of Modern Separation Science
[4] Shaanxi Key laboratory of Modern Separation Science,undefined
[5] Zhejiang Hisun Pharmaceutical Co.,undefined
[6] Ltd.,undefined
来源：
Biotechnology and Bioprocess Engineering | 2013年 / 18卷
关键词：
ion exchange; lysozyme; agarose beads; acrylic acid based resin; thermodynamics and kinetics;
D O I：
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中图分类号：
学科分类号：
摘要：
The present work investigated the adsorption behaviors of lysozyme onto weak cation exchangers at different temperatures. The adsorption isotherm, adsorption thermodynamics and adsorption kinetics were studied. The results indicate that the adsorption of lysozyme onto acrylic acid copolymer based beads (Hydrolite D115) is spontaneous and exothermic, while that onto agarose based beads (CM Sepharose 6 Fast Flow) is also spontaneous, but endothermic. The pseudo second-order kinetic model fits well to the dynamic adsorption experimental data, and the kinetic results are also in concert with the adsorption thermodynamics.
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页码：950 / 955
页数：5
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