Protease production by Streptomyces sp. isolated from brazilian cerrado soilOptimization of culture medium employing statistical experimental design

被引：0
|
作者：
Luciana A. I. de Azeredo
Leda R. Castilho
Selma G. F. Leite
Rosalie R. R. Coelho
Denise M. G. Freire
机构：
[1] Institute of Microbiology Prof. Paulo de Góes,Chemical Engineering Program
[2] COPPE,DBq/Instituto de Química
[3] School of Chemistry,undefined
[4] Universidade Federal do Rio de Janeiro,undefined
[5] Prédio do Centro de Tecnologia,undefined
来源：
Applied Biochemistry and Biotechnology | 2003年 / 108卷 / 1-3期
关键词：
sugarcane molasses; protease production; experimental design;
D O I：
10.1385/ABAB:108:1-3:749
中图分类号：
学科分类号：
摘要：
Streptomyces are important microorganisms because of their capacity to produce numerous bioactive molecules. In the present work protease production, by Streptomyces sp. 594 isolated from a Brazilian Cerrado soil, was maximized by optimizing a low-cost culture medium composition (casitone and sugarcane molasses) using statistical experimental design. The final protease activity (56 U/mL) was 2.8-fold and 58-fold higher than that obtained in the beginning of this study, and in a previous work, using an actinomycete selection medium, respectively. Protease production, not growth associated, appeared to be modulated by an inducer system, whereby the C/N ratio seemed to play a significant role.
引用
收藏
页码：749 / 755
页数：6
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