Protease production by Streptomyces sp. isolated from brazilian cerrado soilOptimization of culture medium employing statistical experimental design

被引：0

作者：

Luciana A. I. de Azeredo

Leda R. Castilho

Selma G. F. Leite

Rosalie R. R. Coelho

Denise M. G. Freire

机构：

[1] Institute of Microbiology Prof. Paulo de Góes,Chemical Engineering Program

[2] COPPE,DBq/Instituto de Química

[3] School of Chemistry,undefined

[4] Universidade Federal do Rio de Janeiro,undefined

[5] Prédio do Centro de Tecnologia,undefined

来源：

Applied Biochemistry and Biotechnology | 2003年 / 108卷 / 1-3期

关键词：

sugarcane molasses; protease production; experimental design;

D O I：

10.1385/ABAB:108:1-3:749

中图分类号：

学科分类号：

摘要：

Streptomyces are important microorganisms because of their capacity to produce numerous bioactive molecules. In the present work protease production, by Streptomyces sp. 594 isolated from a Brazilian Cerrado soil, was maximized by optimizing a low-cost culture medium composition (casitone and sugarcane molasses) using statistical experimental design. The final protease activity (56 U/mL) was 2.8-fold and 58-fold higher than that obtained in the beginning of this study, and in a previous work, using an actinomycete selection medium, respectively. Protease production, not growth associated, appeared to be modulated by an inducer system, whereby the C/N ratio seemed to play a significant role.

引用

页码：749 / 755

页数：6

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