Mass propagation of conifer trees in liquid cultures–progress towards commercialization

被引：6
|
作者：
Pramod K Gupta
Roger Timmis
机构：
[1] Weyerhaeuser Co.,
来源：
Plant Cell, Tissue and Organ Culture | 2005年 / 81卷
关键词：
bioreactor; Douglas-fir; manufactured seed; somatic embryos;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
At Weyerhaeuser Company, somatic embryo production in liquid medium and manufactured seed delivery has been developed to reduce labor costs and increase efficiency of mass clonal propagation. We have scaled-up embryonal suspensor masses (ESM) of Douglas-fir in 1-l liquid medium flasks. Over 10,000 somatic embryos have been produced from a single flask. We have cryostored ESM of over 700 genotypes in liquid nitrogen. Somatic embryos of Douglas-fir have also been produced from liquid medium in a bioreactor. Different types of bioreactors are required for embryo multiplication and for cotyledonary embryo development. Over 250,000 Douglas-fir somatic seedlings from a large number of genotypes have been produced for clonal field tests.
引用
收藏
页码：339 / 346
页数：7
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