Induction of auxin biosynthesis and WOX5 repression mediate changes in root development in Arabidopsis exposed to chitosan

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作者
Federico Lopez-Moya
Nuria Escudero
Ernesto A. Zavala-Gonzalez
David Esteve-Bruna
Miguel A. Blázquez
David Alabadí
Luis V. Lopez-Llorca
机构
[1] Laboratory of Plant Pathology,
[2] Multidisciplinary Institute for Environment Studies (MIES) Ramón Margalef,undefined
[3] Department of Marine Sciences and Applied Biology,undefined
[4] University of Alicante,undefined
[5] Departament d’Enginyeria Agroalimentària i Biotecnologia,undefined
[6] Universitat Politècnica de Catalunya,undefined
[7] Atlántica Agrícola Company SA. Villena,undefined
[8] Instituto de Biología Molecular y Celular de Plantas,undefined
[9] Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia,undefined
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Scientific Reports | / 7卷
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摘要
Chitosan is a natural polymer with applications in agriculture, which causes plasma membrane permeabilisation and induction of intracellular reactive oxygen species (ROS) in plants. Chitosan has been mostly applied in the phylloplane to control plant diseases and to enhance plant defences, but has also been considered for controlling root pests. However, the effect of chitosan on roots is virtually unknown. In this work, we show that chitosan interfered with auxin homeostasis in Arabidopsis roots, promoting a 2–3 fold accumulation of indole acetic acid (IAA). We observed chitosan dose-dependent alterations of auxin synthesis, transport and signalling in Arabidopsis roots. As a consequence, high doses of chitosan reduce WOX5 expression in the root apical meristem and arrest root growth. Chitosan also propitiates accumulation of salicylic (SA) and jasmonic (JA) acids in Arabidopsis roots by induction of genes involved in their biosynthesis and signalling. In addition, high-dose chitosan irrigation of tomato and barley plants also arrests root development. Tomato root apices treated with chitosan showed isodiametric cells respect to rectangular cells in the controls. We found that chitosan causes strong alterations in root cell morphology. Our results highlight the importance of considering chitosan dose during agronomical applications to the rhizosphere.
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