Protective role of antifreeze proteins on sterlet (Acipenser ruthenus) sperm during cryopreservation

被引：0
|
作者：
Miaomiao Xin
Jan Sterba
Anna Shaliutina-Kolesova
Borys Dzyuba
Jaroslava Lieskovska
Serhii Boryshpolets
Mohammad Abdul Momin Siddique
Vitaliy Kholodnyy
Ievgen Lebeda
Otomar Linhart
机构：
[1] University of South Bohemia in Ceske Budejovice,Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology
[2] Chinese Academy of Fishery Sciences,Sino
[3] University of South Bohemia in Ceske Budejovice,Czech Joint Laboratory of Fish Conservation and Biotechnology: Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture of China, Yangtze River Fisheries Research Institute
[4] Institute of Parasitology,Faculty of Science
[5] Noakhali Science and Technology University,Biology Centre, Czech Academy of Sciences
来源：
Fish Physiology and Biochemistry | 2018年 / 44卷
关键词：
Antifreeze proteins; Cryopreservation; Membrane integrity; Motility rate; Sperm quality;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The loss of sperm quality in sterlet (Acipenser ruthenus) due to freeze-thaw process in cryopreservation was investigated in the present study. Two antifreeze proteins (AFPI or AFPIII) were used at different concentrations of 0.1, 1, 10, and 100 μg/mL. We compared motility, curvilinear velocity, and plasma membrane integrity of fresh, cryopreserved sperm, and sperm cryopreserved in the presence of antifreeze proteins. Fresh sperm (control) had 85 ± 4% motility and 160 ± 2 μm/s curvilinear velocity, respectively. After cryopreservation, the motility of frozen-thawed sperm without addition of antifreeze proteins significantly decreased (44 ± 9%), compared to the control. The highest motility of frozen-thawed sperm was obtained in cryopreserved sperm with addition of 1 μg/mL of AFPIII (58 ± 14%). No significant differences were observed in curvilinear velocity between fresh sperm and cryopreserved sperm with/without addition of AFPI or AFPIII. The flow cytometry analysis revealed that fresh sperm contained 94.5 ± 6% live cells, while the cryopreserved sperm only contained 26.6 ± 14% live cells. Supplementation of antifreeze proteins has significantly improved the percentage of live cells in frozen-thawed sperm, except 0.1 μg/ml of AFPI group. No significant difference in percentage of live cells was detected in the sperm cryopreserved with 10 μg/mL of AFPI or AFPIII, compared to fresh sperm. Thus, addition of antifreeze proteins to cryopreservation medium could be considered to improve the post-thawed sperm quality of sterlet.
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页码：1527 / 1533
页数：6
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