A bioreducible polypeptide for efficient gene transfection both in vitro and in vivo

被引：0
|
作者：
Wei Qu
Si Chen
Shan Ren
Xue-jun Jiang
Ren-xi Zhuo
Xian-zheng Zhang
机构：
[1] Wuhan University,Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry
[2] Renmin Hospital of Wuhan University,Department of Cardiology
来源：
Chinese Journal of Polymer Science | 2013年 / 31卷
关键词：
Gene vector; Polypeptide; Angiogenesis; Limb ischemia;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A disulfide bond containing polypeptide, PolyK6-R8, was designed and prepared by oxidative polymerization between terminal cysteinyl thiol groups of CHK6HC and CR8C. The molar ratio between CHK6HC and CR8C within obtained PolyK6-R8 was 9:1, and PolyK6-R8 could combine DNA compactly when weight ratio reached 5. Through in vitro investigation, it was found that PolyK6-R8 was an efficient gene vector with low cytotoxicity for delivering DNA in both COS-7 and HeLa cells. Cellular uptake of DNA mediated by PolyK6-R8/DNA complexes was promoted after incubation for 4 h. Moreover, by examining the histological sections of hindlimb ischemia rats through immunohistochemistry, PolyK6-R8/VEGF complexes were proved to be effective in both VEGF protein expression and succeeding vessel formation. The results indicated that polypeptide-based PolyK6-R8 is a promising gene vector for the limb ischemia treatment.
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页码：713 / 718
页数：5
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